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Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

DOI:
10.3791/62844-v

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11:02 min

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February 12, 2022

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1Department of Biology,Rhode Island College, 2Department of Pediatrics, Women and Infants Hospital,Warren Alpert Medical School of Brown University

Chapitres

  • 00:01Introduction
  • 01:23Initial Fixation of Tissues
  • 03:13Removing Leg Cuticle and Antibody Staining
  • 06:58Mounting Legs
  • 07:41Résultats
  • 10:37Conclusion

Summary

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We describe a dissection technique that preserves the architecture of the neuromuscular junction and enables a detailed immunocytochemical study of motor neurons in the adult Drosophila leg.

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Keywords: DrosophilaAdult LegDissectionImmunohistochemistryNeuromuscular JunctionMotor NeuronNeuronal ArchitectureMuscle ArchitectureDegenerative ChangesGenotypesDisease ModelsAntibodiesMethanolPBSFixationForcepsVannas ScissorsSilicone Elastomer24-well PlateFA SolutionNutator

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