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生物发光光遗传学2.0：利用生物发光在体外和体内激活光敏蛋白
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
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生物发光光遗传学2.0：利用生物发光在体外和体内激活光敏蛋白

DOI:
10.3791/62850-v

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07:19 min

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August 04, 2021

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, , ,
1College of Medicine,Central Michigan University, 2Biochemistry, Cell and Molecular Biology Program,Central Michigan University, 3Neuroscience Program,Central Michigan University

Chapitres

  • 00:04Introduction
  • 00:40Bioluminescence Activation In Vitro
  • 03:26Bioluminescence Activation In Vivo
  • 04:49Results: Bioluminescence for Optogenomic Signaling, Transcription, and Manipulation
  • 06:34Conclusion

Summary

Traduction automatique

Traduction automatique

由氧化小分子底物的荧光素酶发出的生物发光 - 荧光素 - 可以用来激活光敏蛋白，从而为光刺激增加另一个维度，并能够在时间和空间尺度上操纵细胞中多种光介导的功能。

Tags

BioluminescenceOptogeneticsPhotosensory ProteinsIn VitroIn VivoLuciferinCoelenterazine (CTZ)Cell CultureLive Cell ImagingRepeated Light StimulationPerfusion System

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