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リセートベースの無細胞システムにおける代謝物プロファイリングのためのリキッドクロマトグラフィー結合と屈折率または質量分析法検出
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
DOI:

14:42 min

September 23, 2021

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Chapitres

  • 00:04Introduction
  • 01:08Starting, Stopping, and Processing Time Course CFME Reactions for HPLC-RID Quantification
  • 03:18Creating a Sequence Table for Autosampling and Start the HPLC-RID System for Data Acquisition
  • 04:54Extracting and Analyzing Data Post-Run
  • 07:20Starting, Stopping, and Processing Time Course Isotope Tracing CFME Reactions for LC-MS/MS Quantification
  • 09:20Setting Up a Run Sequence and Starting the LC-MS/MS Run
  • 10:16Calculating Negative Mode Masses of 13C-Labeled Glucose-Derived Metabolites and Searching for the m/z Features of These Analytes in Filtered Data
  • 11:45Results: Metabolite Profiling in Lysate-based Cell-free Systems
  • 13:54Conclusion

Summary

Traduction automatique

プロトコルは、複雑な溶解物ベースの無細胞系における代謝反応を研究するための屈折率または質量分析検出に結合された高速液体クロマトグラフィー法を記述する。

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