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Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal
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1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London
Chapitres
- 00:04Introduction
- 00:38SPECT Camera Preparation and Calibration
- 01:47[125I]CLINDE Radiotracer Synthesis
- 04:27SPECT Acquisition
- 05:15Scan Reconstruction
- 05:57Rat Brain Extraction and Cell Isolation
- 09:46Results: FACS-RTT to Determine the Cellular Origin of Radioactive Signal
- 11:27Conclusion
Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) is a powerful tool to study the role of the 18 kDa translocator protein or Serotonin 5HT2A-receptor expression in Alzheimer's Disease at a cellular scale. This protocol describes the ex-vivo application of FACS-RTT in the TgF344-AD rat model.
