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Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells
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Journal JoVE Biologie
Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

DOI:
10.3791/62902-v

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06:17 min

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August 13, 2021

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, , , , ,
1Department of Physiology, Faculty of Medicine in Hradec Králové,Charles University, 2Department of Pathophysiology, Third Faculty of Medicine,Charles University, 3Department of Internal Medicine,University Hospital Kralovske Vinohrady

Chapitres

  • 00:04Introduction
  • 01:03Day 1
  • 03:00Day 2
  • 04:57Results: Mitochondrial Respiration Measurement
  • 05:33Conclusion

Summary

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Traduction automatique

In this work, we describe a modified protocol to test mitochondrial respiratory substrate flux using recombinant perfringolysin O in combination with microplate-based respirometry. With this protocol, we show how metformin affects mitochondrial respiration of two different tumor cell lines.

Tags

Keywords: Mitochondrial Substrate FluxRecombinant Perfringolysin O-permeabilized CellsCancer Cell ResponseMetformin TreatmentMitochondrial MetabolismSeahorse XF 96 Cell Culture MicroplateOligomycinFCCPRotenone Antimycin A

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