Journal
/
Neurosciences
/
Live-imaging af Mitokondrie System i kultiverede astrocytter
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Live-imaging of Mitochondrial System in Cultured Astrocytes

Live-imaging af Mitokondrie System i kultiverede astrocytter

DOI:
10.3791/62957-v

•

06:20 min

•

November 16, 2021

•

, , ,
1Department of Psychiatry, Center for Psychiatric Neurosciences,Lausanne University Hospital (CHUV) and University of Lausanne

Chapitres

  • 00:04Introduction
  • 00:44Rat Hippocampal Astrocyte Primary Culture
  • 01:25Long-Term Monitoring of the Mitochondrial System
  • 02:13Analysis of Individual Mitochondrial Morphology and LV-G1-MitoTimer Ratio
  • 03:54Analysis of Mitochondrial Motility
  • 04:28Data Transformation, Normalization, and Statistical Analysis
  • 04:51Results: Analysis of the Effects of H2O2 on the Mitochondrial System of Astrocytes
  • 05:53Conclusion

Summary

Traduction automatique

Traduction automatique

I denne artikel beskrives metoden til mitokondrietidsfordærv billeddannelse af astrocytkulturer udstyret med MitoTimer biosensor og den resulterende multiparametriske analyse af mitokondriedynamik, mobilitet, morfologi, biogenese, redox-tilstand og omsætning.

Tags

Keywords: Live-imagingMitochondrial SystemCultured AstrocytesMicroscopyMitochondrial DynamicsLentiviral VectorMitoTimerFluorescence ImagingImage ProcessingSegmentationMitochondrial Measurements

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/62957?article_type=v

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
check_url/fr/62957?article_type=v

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
check_url/fr/62957?article_type=v

Neuromodulation and Mitochondrial Transport: Live Imaging in Hippocampal Neurons over Long Durations
check_url/fr/62957?article_type=v

Live Imaging of Dorsal Root Axons after Rhizotomy
check_url/fr/62957?article_type=v

Høj opløsning Live-Imaging af celle adfærd i udviklingslandene neuroepitel
check_url/fr/62957?article_type=v

Ex vivo Live-Imaging af Single celledelinger i Mouse neuroepithelium
check_url/fr/62957?article_type=v

Live-Imaging mitosens i udviklingslandene mus embryonale Cortex
check_url/fr/62957?article_type=v

Live-Imaging af<em> Drosophila</em> Larvetilstand neuroblasts
check_url/fr/62957?article_type=v

Live-Imaging af ependymale Cilia i laterale ventrikler af Mouse Brain
check_url/fr/62957?article_type=v

Protokol til tredimensionel Konfokal Morfometrisk Analyse af Astrocytter

Read Article