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Generation af menneskelige motorenheder med funktionelle neuromuskulære vejkryds i mikrofluidiske enheder
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1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute,KU Leuven – University of Leuven, 2VIB Bio Imaging Core,KU Leuven – University of Leuven, 3Department of Development and Regeneration, Stem Cell and Developmental Biology,KU Leuven – University of Leuven, 4Department of Neurology,University Hospitals Leuven
Chapitres
- 00:05Introduction
- 00:54Pre-Assembled Microfluidic Device Preparation
- 02:39Silicone Microfluidic Device Preparation
- 05:18Neural Progenitor Cell Plating in Microfluidic Devices
- 07:15Human Primary Mesoangioblast (MAB) Plating in Microfluidic Devices
- 08:32Results: Generation of NMJs in Microfluidic Devices
- 10:10Conclusion
Vi beskriver en metode til at generere menneskelige motorenheder i kommercielt tilgængelige mikrofluidiske enheder ved at co-culturing human induceret pluripotente stamcelle-afledte motor neuroner med menneskelige primære mesoangioblast-afledte myotubes resulterer i dannelsen af funktionelt aktive neuromuskulære knudepunkter.
