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Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis
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Neurosciences
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Journal JoVE Neurosciences
Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

Two Peeling Methods for the Isolation of Photoreceptor Cell Compartments in the Mouse Retina for Protein Analysis

DOI:
10.3791/62977-v

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11:08 min

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December 07, 2021

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, ,
1Zilkha Neurogenetic Institute, Keck School of Medicine,University of Southern California, 2Department of Cell & Neurobiology, Keck School of Medicine,University of Southern California

Chapitres

  • 00:04Introduction
  • 00:52Live Cell Retinal Peeling Method
  • 03:35Lyophilized Retina Peeling Method
  • 07:42Results: Isolation of Subcellular Compartments of Murine Rod Photoreceptors for Protein Analysis
  • 10:23Conclusion

Summary

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Traduction automatique

This protocol presents two techniques to isolate the subcellular compartments of murine rod photoreceptors for protein analysis. The first method utilizes live retinae and cellulose filter paper to separate rod outer segments, while the second employs lyophilized retinae and adhesive tape to peel away rod inner and outer segment layers.

Tags

Keywords: Photoreceptor Cell CompartmentsMouse RetinaProtein AnalysisPeeling TechniquesRod Subcellular CompartmentsRod Outer Segment (ROS)Ames HEPES BufferFilter PaperC57 Black 6J MouseRetinal DissectionProtein Analysis

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