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Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability
DOI:

07:23 min

August 06, 2021

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Chapitres

  • 00:05Introduction
  • 00:45Laser Setup
  • 02:05Mouse Tail Vein Injection of Rose Bengal
  • 02:54Occlusion of Major Veins
  • 04:07Establishing the Number of Veins Occluded at Day 0 and Aftercare
  • 04:40Results: Analysis of Types and Timelines of Photo-Occlusion, Baseline and Experimental Laser Output Measurement, Ophthalmic Pathologies, and Quantification of OCT Images
  • 06:37Conclusion

Summary

Traduction automatique

Here, we describe an optimized protocol for retinal vein occlusion using rose bengal and a laser-guided retinal imaging microscope system with recommendations to maximize its reproducibility in genetically modified strains.

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