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Automatisering af aggregeret kvantificering i Caenorhabditis elegans
Journal JoVE
Biologie
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Journal JoVE Biologie
Automating Aggregate Quantification in Caenorhabditis elegans

Automatisering af aggregeret kvantificering i Caenorhabditis elegans

DOI:
10.3791/62997-v

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07:50 min

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October 14, 2021

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, , , , ,
1Department of Microbiology and Cell Science,University of Florida, 2Department of Computer and Information Science and Engineering,University of Florida

Chapitres

  • 00:04Introduction
  • 02:28Image Analysis
  • 05:38Results: The Analysis of Aggregate Detection and the Aggregate Quantification Efficacy Using CellProfiler and Automation

Summary

Traduction automatique

Traduction automatique

Følgende protokol beskriver udviklingen og optimeringen af en arbejdsgang med høj kapacitet til ormedyrkning, fluorescensbilleddannelse og automatiseret billedbehandling for at kvantificere polyglutaminaggregater som en vurdering af ændringer i proteostase.

Tags

Keywords: Polyglutamine AggregationProteostasisCaenorhabditis ElegansAutomated Aggregate CountingHigh-throughputMicroscopy ImagingCellProfilerImage Analysis PipelineUntangled Worms ModuleBacterial GenesNeurodegenerative Diseases

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