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Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
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Journal JoVE Biologie
Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

DOI:
10.3791/63013-v

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08:24 min

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September 27, 2021

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, , , , , ,
1Department of Chemistry,The University of British Columbia, 2Biochemistry and Molecular Biology,The University of British Columbia, 3Faculty of Medicine,The University of British Columbia

Chapitres

  • 00:04Introduction
  • 00:57Flow Chamber Preparation
  • 01:52Surface Preparation
  • 03:14Experiment and Imaging
  • 05:52Results: The Analysis of Image Processing, Suboptimal and Optimal Results, and Bioconjugation Characterization
  • 07:35Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol presents the experimental procedures to perform the adhesion footprint assay to image the adhesion events during fast cell rolling adhesion.

Tags

Keywords: Imaging Molecular AdhesionCell RollingAdhesion Footprint AssayMolecular ForceSurface FunctionalizationBioconjugationFlow ChamberEpoxy-coated TapeWash BufferBSATween 20StreptavidinProtein G-TGT

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