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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
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Journal JoVE Biochimie
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

DOI:
10.3791/63015-v

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04:22 min

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November 20, 2021

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, , , ,
1School of Molecular Sciences,Arizona State University, 2Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, 3Eyring Materials Center,Arizona State University, 4Department of Microbiology and Immunology,The Pennsylvania State University

Summary

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Lipid monolayers have been used as a foundation for forming two-dimensional (2D) protein crystals for structural studies for decades. They are stable at the air-water interface and can serve as a thin supporting material for electron imaging. Here we present the proven steps on preparing lipid monolayers for biological studies.

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Keywords: Lipid MonolayerElectron Crystallography2D CrystallizationMembrane ProteinsProtein BindingSample PreparationTeflon PlateBuffer ReservoirHumidity ControlCarbon-coated EM Grid

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