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Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
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Journal JoVE Biologie
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

DOI:
10.3791/63020-v

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07:23 min

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September 14, 2021

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, , ,
1Vector-borne Virus Research Center, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology,Fujian Agriculture and Forestry University

Chapitres

  • 00:05Introduction
  • 00:35Non-Viruliferous Insect Rearing
  • 01:29Virus Acquisition and Collection of Viruliferous Insects
  • 02:10Collection of Salivary Proteins Using a Feeding Cage
  • 03:04Reagent Preparation
  • 03:51Detection of Saliva and Viral Proteins by Western Blotting
  • 06:02Results: Western Blot Analysis for Detection of Viral and Salivary Proteins of a Leafhopper Vector in the Plant Host
  • 06:51Conclusion

Summary

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Traduction automatique

This protocol demonstrates how to use the plant host to detect salivary proteins of leafhopper and plant viral proteins released by leafhopper vectors.

Tags

Keywords: LeafhopperSalivary ProteinsPlant HostVirus DetectionVirus TransmissionRice Dwarf VirusFeeding CageAspiratorNon-viruliferous NymphsCirculative TransmissionInsect-proof NetTris-Glycine Buffer

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