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In Situ Visualisering af axonvækst og vækstkegledynamik i akutte ex vivo embryonale hjerneskivekulturer
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures
DOI:

10:45 min

October 14, 2021

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Chapitres

  • 00:05Introduction
  • 01:11Embryo Extraction
  • 01:44Ex Utero and In Utero Electroporation
  • 03:35Brain Extraction and Embedding in Agarose
  • 04:39Organotypic Slice Culture
  • 05:43Image Acquisition and Data Analysis
  • 08:29Results: In Situ Axon Growth and Growth Cone Dynamics
  • 09:56Conclusion

Summary

Traduction automatique

Denne protokol demonstrerer en ligetil og robust metode til at studere in situ axon vækst og vækstkegle dynamik. Den beskriver, hvordan man forbereder ex vivo fysiologisk relevante akutte hjerneskiver og giver en brugervenlig analysepipeline.

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