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1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, 2Department of Laboratory Medicine,National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University
Chapitres
- 00:04Introduction
- 01:28DNA Glycosylase Assay
- 04:43Transferring UDG Reaction Products to a Matrix Chip
- 05:33Setting up the Assay Parameters on the Mass Spectrometer
- 07:19Mass Spectrometer Operation, Viewing Mass Spectra, and Analyzing the Data
- 09:03Results: UDG Assay Using MALDI-TOF MS for AP Site Containing Product Analysis
- 11:01Conclusion
En ikke-merket, ikke-radio-isotopisk metode for å analysere uracil-DNA glykosylaseaktivitet ble utviklet ved hjelp av MALDI-TOF massespektrometri for direkte apurinic / apyrimidinisk produktanalyse. Analysen viste seg å være ganske enkel, spesifikk, rask og enkel å bruke til DNA glykosylasemåling.
