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1School of Women’s and Children’s Health, Faculty of Medicine and Health,University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health,University of New South Wales, 3Department of Respiratory Medicine,Sydney Children’s Hospital, 4Department of Pathology, School of Medical Sciences,University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre,University of New South Wales
Chapitres
- 00:05Introduction
- 01:16Collection of Nasal Inferior Turbinate Brushings
- 02:25Airway Epithelial Cell Expansion and Maintenance
- 03:23Seeding and Differentiation of Airway Epithelial Cells and Maintenance of Differentiated ALI Models
- 04:12Three-Dimensional Airway Epithelial Organoids
- 05:54Imaging Cilia Beat Frequency
- 07:18Data Analysis and Quantification of Cilia Beat Frequency
- 09:20Results: Cilia Beat Frequency in Airway Epithelial Cell ALI Models of Cystic Fibrosis Patients and Healthy Participants
- 10:36Conclusion
Este protocolo descreve a coleta, expansão e diferenciação de células epiteliais nasais para modelos de células epiteliais organotípicas das vias aéreas e quantificação da frequência de batimentos de cílios por meio de imagens de células vivas e scripts personalizados.
