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从人牙囊中分离上皮细胞

Journal JoVE
Biologie

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Journal JoVE Biologie

Isolation of Epithelial Cells from Human Dental Follicle

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从人牙囊中分离上皮细胞

DOI:

10.3791/63104-v

•

04:07 min

•

November 05, 2021

•

Hyosun Jung, Jin-Kyu Yi²

¹Department of Conservative Dentistry,Kyung Hee University Dental Hospital at Gangdong, ²Department of Conservative Dentistry, School of Dentistry,Kyung Hee University

Chapitres

00:04Introduction
00:34Isolate Single-Cell Populations from Dental Follicle
02:52Results: Emergence of Epithelial Cells
03:13Conclusion

Summary

Traduction automatique

牙囊含有上皮群和间充质细胞。通过提供独特的培养基，从异质性牙卵泡细胞群中选择上皮群体。上皮细胞存活并在无血清培养基中形成菌落。

Tags

Dental Follicle Epithelial Cells Cell Isolation Cell Culture Collagenase Protease Trypsin-EDTA Keratinocyte Medium Cell Strainer Centrifugation Cell Morphology Cell Colonies

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