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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Médecine
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Journal JoVE Médecine
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

DOI:
10.3791/63107-v

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08:23 min

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February 16, 2022

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, , ,
1Department of Anesthesia, Critical Care and Pain Medicine,Massachusetts General Hospital, 2Department of Pediatrics,Massachusetts General Hospital, 3Harvard Medical School

Chapitres

  • 00:04Introduction
  • 01:07Setup Preparation for Nitric Oxide Consumption by Cell-Free Hemoglobin
  • 01:33Starting the Chemiluminescence Detector and Preparing the Purge Vessel
  • 03:56Performing the Experiment
  • 05:57Results: Nitric Oxide-Consumption by Cell-Free Hemoglobin
  • 07:57Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present protocols for detecting nitric oxide and its biologically relevant derivatives using chemiluminescence-based assays with high sensitivity.

Tags

Keywords: ChemiluminescenceNitric OxideDETA-NONOateOxygenPurge VesselInert GasMetabolitesAssayDetectionTherapeutic GasAntimicrobialAirway Infectious DiseasesLiquid Phase Assays

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