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Preparazione di doppi strati lipidici supportati da perline per studiare la produzione di particolato delle sinapsi immunitarie delle cellule T
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Chapitres
- 00:04Introduction
- 01:07Protein Density Calibrations on BSLBs
- 01:47Titration of His-Tagged Proteins on 12.5 mol% of DGS-(Ni) NTA-Containing BSLBs
- 04:02Measurement of T-cell Output Transfer to BSLBs
- 08:13Results: FCM for Absolute Protein Quantification on the Cell Surface
- 10:17Conclusion
Tags
Keywords:
Bead-supported Lipid BilayersImmune SynapseFlow CytometryMicroscopyProteomicsRNA SequencingChimeric Antigen ReceptorsSignaling PathwaysGenetic AblationProtein CalibrationMulticolor Flow CytometrySilica BeadsLiposomeNickel-containing PhospholipidsArgonNitrogenBlockingBSANickel SulfateHBS-HSA BufferProtein Dilutions
