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Biologie du développement
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Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

DOI:
10.3791/63148-v

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10:49 min

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February 14, 2022

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, , ,
1Department of Cell and Molecular Biology,Karolinska Institutet

Chapitres

  • 00:05Introduction
  • 00:55Needle Loading
  • 01:38Injections
  • 07:53Results: Transduction of the Murine Neural Plate by Nano-Injection at Embryonic Day 7.5
  • 10:07Conclusion

Summary

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Traduction automatique

In this protocol, we describe how to inject the mouse amniotic cavity at E7.5 with lentivirus, leading to uniform transduction of the entire neural plate, with minimal detrimental effects on survival or embryonic development.

Tags

Keywords: NEPTUNEMurine Neural Plate TargetingIn Utero Nano-injectionEmbryonic Day 7.5Genetic ManipulationKnockdownLineage TracingShRNACdRNABarcoded Viral LibrariesNeurosciencesOrgan SystemsAmniotic CavityUltrasoundGlass NeedleMetal PlungerCapillary NeedleOilSolutionAnesthetized Female MousePainkillerChlorhexidine SolutionSkin IncisionMuscle LayerUterine HornsPBSElastic Membrane

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