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Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

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Biochimie
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Journal JoVE Biochimie

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

DOI:

10.3791/63164-v

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14:39 min

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April 22, 2022

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Óscar A. Muñoz-Bernal, Alma A. Vazquez-Flores, Emilio Alvarez-Parrilla, Nina R. Martínez-Ruiz, Laura A. de la Rosa

¹Department of Chemical Biological Sciences,Universidad Autónoma de Ciudad Juárez

Chapitres

00:42Introduction
02:58Sample Preparation
04:37UPLC-MS/MS Analysis
06:36Database Construction
09:49Data Analysis
11:25Representative Results
14:13Conclusions

Summary

Traduction automatique

The goal of this protocol is to detect phenolic metabolites in plasma using a semi-targeted chromatography-mass spectrometry method.

Tags

Keywords: Semi-targeted Ultra-high-performance Chromatography Mass Spectrometry Phenolic Metabolites Plasma Elderly Adults UPLC-MS/MS Protein Precipitation Metabolite Identification Brosimum Alicastrum Bioavailability Biotransformation Untargeted Analysis Semi-targeted Analysis Molecular Features Mass-to-charge Ratio Isotopic Distribution Fragmentation Pattern Polyphenol Metabolites

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