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Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle
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Journal JoVE Biologie
Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle

Preparing and Rearing Axenic Insects with Tissue Cultured Seedlings for Host-Gut Microbiota Interaction Studies of the Leaf Beetle

DOI:
10.3791/63195-v

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06:56 min

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October 08, 2021

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, , , ,
1State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences,Hubei University, 2Institute of Plant Protection,Wuhan Institute of Landscape Architecture, 3McKetta Department of Chemical Engineering,University of Texas at Austin

Chapitres

  • 00:05Introduction
  • 00:54Insect Rearing
  • 01:43Germ-Free Poplar Culturing
  • 02:40Egg Surface Sterilization and Rearing Axenic Larvae
  • 03:47Verification of Axenic Larvae with Culture-Dependent Assays
  • 04:40Verification of Axenic Individuals with Culture-Independent Assays
  • 05:32Results: Confirmation of Efficacy of Gut Bacteria Elimination
  • 06:07Conclusion

Summary

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Traduction automatique

To obtain an axenic insect, its egg surface is sterilized, and the hatched larva is subsequently reared using axenic leaves. This method provides an efficient way for axenic insect preparation without administering antibiotics or developing an artificial diet, which can also be applied to other leaf-eating insects.

Tags

Keywords: Axenic InsectsGut MicrobiotaLeaf BeetlePlant SeedlingsAlpha-naphthalene Acetic AcidMS MediumSurface SterilizationGerm-freeArtificial DietInsect Rearing

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