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使用微图案弹性体对单细胞收缩力进行简化的高通量分析
Journal JoVE
Bio-ingénierie
Author Produced
This content is Free Access.
Journal JoVE Bio-ingénierie
Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
DOI:

14:33 min

April 08, 2022

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Chapitres

  • 00:06Introduction
  • 02:01Day 1: Preparation of the 24-Well Plate
  • 03:16Day 1: Cell Seeding
  • 03:58Day 2: Addition of Test Drug
  • 06:17Day 2: Imaging the Well-Plate
  • 06:48Post-Experiment: Image Analysis
  • 10:27Representative Results
  • 12:22Discussion

Summary

Traduction automatique

这项工作提出了一种灵活的方案,用于利用微孔格式的荧光标记弹性收缩表面(FLECS)技术,基于荧光蛋白微模式的可视化位移,对单细胞收缩力进行简化,无需干预的定量。

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