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Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
DOI:

07:02 min

December 16, 2021

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Chapitres

  • 00:05Introduction
  • 00:58Preliminary Sample Preparation
  • 02:12Protein Hydrolysis
  • 03:21Manual Solid-Phase Extraction
  • 04:21Robotic Solid Phase Extraction for NMR Sample Preparation
  • 04:59Results: Measurement of Arginine Methylome by NMR Spectroscopy
  • 05:55Conclusion

Summary

Traduction automatique

The present protocol describes the preparation and quantitative measurement of free and protein-bound arginine and methyl-arginines by 1H-NMR spectroscopy.

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