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Purification of Endogenous Drosophila Transient Receptor Potential Channels

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Biochimie

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Journal JoVE Biochimie

Purification of Endogenous Drosophila Transient Receptor Potential Channels

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Purification of Endogenous Drosophila Transient Receptor Potential Channels

DOI:

10.3791/63260-v

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08:39 min

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December 28, 2021

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Jia Liu*¹, Yuyang Liu*¹, Weidi Chen, Yuzhen Ding, Xiaoru Lan, Wei Liu

¹Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute,Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center

Chapitres

00:04Introduction
00:53Purification of GST-Tagged TRP and His-Tagged NORPA Fragment
03:47Preparation of Drosophila Heads
04:22Drosophila TRP Channel Purification
06:15Size-Exclusion Column Purification of DrosophilaTRP Channel
06:35Results: Purification of Endogenous Drosophila TRP Channel
07:30Conclusion

Summary

Traduction automatique

Based on the assembling mechanism of the INAD protein complex, in this protocol, a modified affinity purification plus competition strategy was developed to purify the endogenous Drosophila TRP channel.

Tags

Keywords: Drosophila Transient Receptor Potential (TRP) Channels Protein Purification GST-tagged TRP Size Exclusion Chromatography E. Coli Expression INAD Protein Complex High-pressure Homogenization

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