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1Division of Parasitic Diseases and Malaria, Entomology Branch,Centers for Disease Control and Prevention, 2Department of Entomology,Cornell University, 3Centro de Estudios en Biotecnologia,Universidad del Valle de Guatemala, 4Department of Cell Biology,Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute,Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology,Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology,Johns Hopkins School of Medicine, 8Biomedical Sciences Department,Idaho College of Osteopathic Medicine
Chapitres
- 00:05Introduction
- 00:45Preparation of Heat-Killed Bacteria Expressing Double-Stranded RNA (dsRNA)
- 02:09Feeding Mosquitoes with Heat-Killed Bacteria Expressing dsRNA
- 02:47Assay Target Gene Expression Levels
- 03:59Phenotypic Evaluation: Successful Blood-Feeding
- 04:35Phenotypic Evaluation: Salivary Gland Morphology and Down-Regulation of Relevant Proteins
- 05:30Results: The Effects of fkh Knockdown in Adult Anopheles gambiae Salivary Glands
- 07:17Conclusion
Die orale Verabreichung von dsRNA, die von Bakterien produziert wird, eine Verabreichungsmethode für RNA-Interferenz (RNAi), die routinemäßig bei Caenorhabditis elegans angewendet wird, wurde hier erfolgreich auf erwachsene Moskitos angewendet. Unsere Methode ermöglicht robuste Studien zur Umkehrgenetik und übertragungsblockierende Vektorstudien ohne den Einsatz von Injektionen.
