Spinal Cord Transection In Xenopus laevis Tadpoles

DOI:

10.3791/63276-v

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05:54 min

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December 10, 2021

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Paula G. Slater, Juan Larraín

¹Center for Aging and Regeneration, Facultad de Ciencias Biológicas,P. Universidad Católica de Chile

Chapitres

00:04Introduction
01:07Staging
01:39Surgery: Spinal Cord Transection and Sham-Operated Animals
02:42Post-Surgery Care
03:32Swimming Assay
04:26Results: Swimming Recovery After Spinal Cord Injury in Xenopus laevis Tadpoles
05:24Conclusion

Summary

Traduction automatique

Xenopus laevis tadpole spinal cord transection is a relevant injury method to study spinal cord injury and regeneration by making a transverse cut that completely severs the spinal cord at the thoracic level.

Spinal Cord Transection In Xenopus laevis Tadpoles

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Chapitres

Summary

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Vidéos Connexes

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Obtaining Eggs from Xenopus laevis Females

Preparation and Fractionation of Xenopus laevis Egg Extracts

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Spinal Cord Electrophysiology

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

Live-cell Imaging and Quantitative Analysis of Embryonic Epithelial Cells in Xenopus laevis

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Facial Transplants in Xenopus laevis Embryos

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation

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