/
/
Dyrkning af lymfocytter i simuleret mikrogravitation ved hjælp af et roterende cellekultursystem
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine,University of Toronto, 2Krembil Research Institute, Toronto Western Hospital,University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital,Sinai Health Systems, 4Medical Oncology and Hematology,Princess Margaret Cancer Centre
Chapitres
- 00:04Introduction
- 00:48Preparation of Vessels for Cell Culture
- 01:43Loading Vessels with Stock Cell Culture
- 04:42Removing Bubbles from the Vessels
- 05:47Attaching the Vessel to the Rotating Base
- 06:36Treatment and Harvesting Cells from the Vessels
- 08:06Results: Application of the Rotary Cell Culture System to Study the Effects of Simulated Microgravity on Lymphocytes
- 08:55Conclusion
Dette er en trinvis vejledning til brug af et kommercielt tilgængeligt roterende cellekultursystem til dyrkning af lymfocytter i simuleret mikrogravitation ved hjælp af specialiserede engangskulturbeholdere. Denne dyrkningsmetode kan anvendes på enhver cellekultur af suspensionstypen.
Tags
LymphocytesSimulated MicrogravityRotary Cell Culture SystemImmune CellsSpace EnvironmentHuman PhysiologyMolecular LevelEconomically FeasibleCell CultureControlTreatmentComplete MediaIncubatorFlask ControlT25 Suspension Culture FlaskStock Cell CultureSyringe Port CapsStopcocksFill PortSerological PipetteVacuum System
