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Dyrking av lymfocytter i simulert mikrogravitasjon ved hjelp av et roterende cellekultursystem
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1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine,University of Toronto, 2Krembil Research Institute, Toronto Western Hospital,University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital,Sinai Health Systems, 4Medical Oncology and Hematology,Princess Margaret Cancer Centre
Chapitres
- 00:04Introduction
- 00:48Preparation of Vessels for Cell Culture
- 01:43Loading Vessels with Stock Cell Culture
- 04:42Removing Bubbles from the Vessels
- 05:47Attaching the Vessel to the Rotating Base
- 06:36Treatment and Harvesting Cells from the Vessels
- 08:06Results: Application of the Rotary Cell Culture System to Study the Effects of Simulated Microgravity on Lymphocytes
- 08:55Conclusion
Dette er en trinnvis veiledning for bruk av et kommersielt tilgjengelig roterende cellekultursystem for å dyrke lymfocytter i simulert mikrogravitasjon ved bruk av spesialiserte engangskulturbeholdere. Denne dyrkingsmetoden kan anvendes på enhver suspensjon-type cellekultur.
Tags
LymphocytesSimulated MicrogravityRotary Cell Culture SystemImmune CellsSpace EnvironmentHuman PhysiologyMolecular LevelEconomically FeasibleCell CultureControlTreatmentComplete MediaIncubatorFlask ControlT25 Suspension Culture FlaskStock Cell CultureSyringe Port CapsStopcocksFill PortSerological PipetteVacuum System
