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在几种肾脏疾病小鼠模型中使用聚乙烯亚胺纳米颗粒将外源人工合成的miRNA模拟物递送到肾脏

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Médecine

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Journal JoVE Médecine

Delivery of Exogenous Artificially Synthesized miRNA Mimic to the Kidney Using Polyethylenimine Nanoparticles in Several Kidney Disease Mouse Models

Please note that all translations are automatically generated. Click here for the English version.

在几种肾脏疾病小鼠模型中使用聚乙烯亚胺纳米颗粒将外源人工合成的miRNA模拟物递送到肾脏

DOI:

10.3791/63302-v

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07:01 min

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May 10, 2022

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Katsunori Yanai*¹, Shohei Kaneko, Hiroki Ishii, Akinori Aomatsu, Yoshiyuki Morishita*¹

¹Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University

Chapitres

00:05Introduction
00:33Preparation of PEI-NPs-miRNA-Mimic Complex
01:43Confirmation of Significant Delivery of miRNA Mimic to the Kidney in UUO Mice by PEI-NP via Tail Vein Injection Using Fluorescent Microscopy
03:48Results: Delivery and Effects of Target miRNAs Using PEI-NPs in Renal Fibrosis Mice, Diabetic Kidney Disease Model Mice, and Acute Kidney Injury Model Mice Produced by IRI
06:33Conclusion

Summary

Traduction automatique

在这里，我们通过在几种肾脏疾病小鼠模型中通过尾静脉注射非病毒载体和聚乙烯亚胺纳米颗粒将外源人工合成的miRNA模拟物输送到肾脏。这导致靶miRNA在肾脏中的显着过表达，导致几种小鼠模型中肾脏疾病的进展受到抑制。

Tags

Keywords: MiRNA Mimic Polyethylenimine Nanoparticles Kidney Disease Mouse Models Fluorescent Labeling Kidney Perfusion Cryosectioning

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