Journal
/
Médecine
/
Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

DOI:
10.3791/63307-v

•

09:45 min

•

March 14, 2022

•

, , , , , ,
1Department of Diabetes Complications and Metabolism,City of Hope, 2Department of Bioengineering,University of California San Diego, 3Irell and Manella Graduate School of Biological Sciences,City of Hope

Chapitres

  • 00:04Introduction
  • 01:28Physical Dissociation
  • 03:26Preparation for RNA-Seq Studies
  • 04:28Spatial Transcriptome Profiling
  • 07:13Results: Analysis of Endothelial Cells (ECs)
  • 08:52Conclusion

Summary

Traduction automatique

Traduction automatique

The protocol describes the isolation, culture, and profiling of endothelial cells from human mesenteric artery. Additionally, a method is provided to prepare human artery for spatial transcriptomics. Proteomics, transcriptomics, and functional assays can be performed on isolated cells. This protocol can be repurposed for any medium- or large-size artery.

Tags

Keywords: Endothelial CellsMesenteric ArteryTranscriptomeSingle-cellSpatial ResolutionDiabetesObesityCryostat SectioningCell IsolationDigestion BufferCell Culture

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/63307?article_type=v

Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
check_url/fr/63307?article_type=v

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
check_url/fr/63307?article_type=v

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
check_url/fr/63307?article_type=v

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
check_url/fr/63307?article_type=v

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
check_url/fr/63307?article_type=v

Isolation and Culture Expansion of Tumor-specific Endothelial Cells
check_url/fr/63307?article_type=v

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
check_url/fr/63307?article_type=v

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
check_url/fr/63307?article_type=v

Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
check_url/fr/63307?article_type=v

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Read Article