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Rask innkapsling av rekonstituert cytoskjelett inne i gigantiske unilamellar vesicles
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1Department of Mechanical Engineering,University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences,Harvard University, 3Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering,University of Michigan, Ann Arbor, 5Department of Biophysics,University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program,University of Michigan, Ann Arbor
Chapitres
- 00:05Introduction
- 00:48Preparation of Oil-Lipid-Mixture
- 02:12Vesicle Generation
- 05:42Imaging and 3D image Reconstruction
- 06:53Results: Encapsulation of Actin Bundle Structures
- 07:08Conclusion
Denne artikkelen introduserer en enkel metode for rask produksjon av gigantiske unilamellar vesikler med innkapslede cytoskeletale proteiner. Metoden viser seg å være nyttig for nedenfra-og-opp-rekonstituering av cytoskeletale strukturer i innesperring og cytoskjelettmembraninteraksjoner.
