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Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique
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Journal JoVE Neurosciences
Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique

Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique

DOI:
10.3791/63338-v

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11:41 min

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April 08, 2022

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, , ,
1Laboratory of Neurochemistry, Institute of Biophysics Carlos Chagas Filho,Universidade Federal do Rio de Janeiro, 2Laboratory of Neuroenergetics and Inborn Errors of Metabolism, Institute of Medical Biochemistry Leopoldo de Meis,Universidade Federal do Rio de Janeiro, 3Laboratory of Neurochemistry and Cell Biology, Institute of Life Sciences,Universidade Federal da Bahia

Chapitres

  • 00:00Introduction
  • 00:59Neurons or Glia?
  • 01:30Retina Dissection and Cell Culture Preparation
  • 04:48Loading Cells with FURA-2 AM
  • 06:21Calcium Imaging of Cells
  • 09:10Data Processing
  • 09:38Representative Results
  • 10:50Conclusion

Summary

Traduction automatique

Traduction automatique

Cell calcium imaging is a versatile methodology to study dynamic signaling of individual cells, on mixed populations in culture or even on awakened animals, based on the expression of calcium-permeable channels/receptors that gives unique functional signatures.

Tags

Keywords: Calcium ImagingNeuron-glia CircuitCalcium SignalingCalcium DyeFura-2 AMPotassium ChlorideATPMembrane PotentialVoltage-gated Calcium ChannelsP2X7 ChannelsGlial CellsChicken Retina CultureDissectionCentrifugationCell Culture

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