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人爆破模拟胚发育和植入的协议

Journal JoVE
Biologie du développement

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Journal JoVE Biologie du développement

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

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人爆破模拟胚发育和植入的协议

DOI:

10.3791/63388-v

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12:09 min

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August 10, 2022

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Harunobu Kagawa*¹, Alok Javali*¹, Heidar Heidari Khoei*¹, Theresa Maria Sommer³, Giovanni Sestini³, Maria Novatchkova, Yvonne Scholte op Reimer, Nicolas Rivron

¹Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA),Vienna Biocenter (VBC), ²Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), ³Vienna BioCenter PhD Program,Doctoral School of the University of Vienna and Medical University of Vienna

Chapitres

00:04Introduction
01:24Formation of Naive PSC Aggregates
04:57Blastoid Development
05:49Formation of Blastoids in 96-well Ultra-low Attachment Microplates
07:05Formation of Trophospheres
08:05Results: Blastoid and Trophospheres Derived from Aggregates of Naïve hPSC
11:20Conclusion

Summary

Traduction automatique

一种概述人胚层形成的方案，可有效，及时和依次产生囊胚样细胞。

Tags

Keywords: Human Blastoids Blastocyst Development Implantation In Vitro Fertilization Pre-clinical Modeling Therapeutic Targets Non-hormonal Contraceptives MEF Exclusion PXGL Media N2B27 Basal Media Aggregation Media Cell Detachment MicroWells

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