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Bio-ingénierie

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经济实惠的氧气显微镜辅助多细胞球体生物制造

Journal JoVE
Bio-ingénierie

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Journal JoVE Bio-ingénierie

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

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经济实惠的氧气显微镜辅助多细胞球体生物制造

DOI:

10.3791/63403-v

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13:21 min

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April 06, 2022

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Irina A. Okkelman*¹, Chris Vercruysse*¹, Alina V. Kondrashina, Sergey M. Borisov, Ruslan I. Dmitriev

¹Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences,Ghent University, ²Health and Happiness (H&H) Group,National Food Innovation Hub, ³Institute of Analytical Chemistry and Food Chemistry,Graz University of Technology

Chapitres

00:04Introduction
00:48Preparing Micro-Patterned Agarose Coated Tissue Culture Plate
02:27Preparing Oxygen Probe-Loaded Spheroids
03:50Spheroids Bioprinting Procedure
05:48Preparation of Spheroids for Live Imaging Analysis
07:10Image Acquisition and Presentation
10:22Results: Kinetic Study of Oxygen Probe and Comparative Analysis of Spheroids
12:18Conclusion

Summary

Traduction automatique

该协议描述了使用标准荧光显微镜上氧化和细胞死亡的多参数分析来生成用于生物打印的高通量球体。这种方法可以应用于控制球体的活力并进行标准化，这对于3D组织建模，肿瘤微环境和成功的（微）组织生物制造非常重要。

Tags

Keywords: Oxygen Microscopy Biofabrication Multicellular Spheroids 3D Printing Near-infrared Oxygen Probe PDMS Stamps Agarose Micro-patterning Cell Culture Spheroid Formation

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