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Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
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Journal JoVE Biologie
Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

DOI:
10.3791/63432-v

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06:39 min

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February 04, 2022

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,
1Faculty of Animal Science and Food Engineering, Department of Basic Sciences,University of São Paulo, 2Laboratory of Nuclear Instrumentation, Center of Nuclear Energy in Agriculture,University of São Paulo

Chapitres

  • 00:04Introduction
  • 00:58Sample Harvesting, Fixation, and Dehydration
  • 01:42Historesin Embedding Procedure
  • 02:23Polymerization
  • 03:24Sectioning and Double Staining Process
  • 04:27Results: Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
  • 05:59Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes a microscopical method to detect pectin in coffee-fungus interaction.

Tags

Keywords: Double-stainingPectinPlant-fungus InteractionHistopathological StudiesCoffee RustCercosporiosisKarnovsky FixativeCacodylate BufferGlycol MethacrylatePolymerization

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