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Dobbeltfarvningsmetode til påvisning af pektin i interaktion mellem plante og svampe

Journal JoVE
Biologie

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Journal JoVE Biologie

Double-Staining Method to Detect Pectin in Plant-Fungus Interaction

Dobbeltfarvningsmetode til påvisning af pektin i interaktion mellem plante og svampe

DOI:

10.3791/63432-v

•

06:39 min

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February 04, 2022

•

João Paulo Rodrigues Marques, Laura Galvan Nuevo

¹Faculty of Animal Science and Food Engineering, Department of Basic Sciences,University of São Paulo, ²Laboratory of Nuclear Instrumentation, Center of Nuclear Energy in Agriculture,University of São Paulo

Chapitres

00:04Introduction
00:58Sample Harvesting, Fixation, and Dehydration
01:42Historesin Embedding Procedure
02:23Polymerization
03:24Sectioning and Double Staining Process
04:27Results: Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
05:59Conclusion

Summary

Traduction automatique

Denne protokol beskriver en mikroskopisk metode til påvisning af pektin i kaffe-svamp interaktion.

Tags

Keywords: Double-staining Pectin Plant-fungus Interaction Histopathological Studies Coffee Rust Cercosporiosis Karnovsky Fixative Cacodylate Buffer Glycol Methacrylate Polymerization

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