/
/
Optrode Array til samtidig optogenetisk modulering og elektrisk neural optagelse
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Electronic and Electrical Engineering,Ewha Womans University, 2Graduate Program in Smart Factory,Ewha Womans University, 3Department of Electrical and Computer Engineering,Seoul National University, 4Graduate School of Engineering Practice,Seoul National University, 5Department of Brain and Cognitive Sciences,Ewha Womans University
Chapitres
- 00:04Introduction
- 01:13Preparation of an Optrode Array
- 01:39Presurgical Preparation of Animal and Implantation Surgery
- 03:45Setting Parameters and Conducting Optogenetic Stimulation, Electrophysiological Recording, and Data Analysis
- 04:55Results: Electrophysiological Recording
- 05:40Conclusion
Her præsenterer vi fremstillingsmetoden for et optrodesystem med optiske fibre til lystilførsel og et elektrodearray til neural optagelse. In vivo-eksperimenter med transgene mus, der udtrykker channelrhodopsin-2, viser systemets gennemførlighed til samtidig optogenetisk stimulering og elektrofysiologisk optagelse.
