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A Human Corneal Organ Culture Model of Descemet’s Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1
Journal JoVE
Médecine
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Journal JoVE Médecine
A Human Corneal Organ Culture Model of Descemet’s Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1

A Human Corneal Organ Culture Model of Descemet’s Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1

DOI:
10.3791/63482-v

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12:36 min

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July 22, 2022

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, , ,
1Trefoil Therapeutics, Inc.

Chapitres

  • 00:16Introduction
  • 02:27Wound Creation
  • 04:12Descemet’s Stripping
  • 05:51Trypan Blue Staining
  • 08:51Representative Results
  • 11:49Conclusion

Summary

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Descemet's Stripping Only is an experimental procedure wherein patients with central corneal guttae resulting from Fuchs Endothelial Corneal Dystrophy have Descemet's membrane stripped for peripheral cells to regenerate the endothelial layer. We present novel methodology simulating DSO in dystrophic human corneas ex vivo with accelerated healing stimulated by eFGF1 (NM141).

Tags

Keywords: Corneal Organ CultureDescemet’s Stripping Only (DSO)Fuch’s Endothelial Corneal Dystrophy (FECD)Engineered Fibroblast Growth Factor 1Endothelial KeratoplastyCorneal Endothelial CellsDescemet’s MembraneCorneal GuttaeWound HealingCorneal Transplantation

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