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Sanntids, tofarget stimulert ramanspredningsavbildning av musehjerne for vevsdiagnose
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Chapitres
- 00:04Introduction
- 00:49Beam Collimation
- 01:32Spatial Overlap
- 02:34Electrooptical Modulation (EOM)
- 03:35Temporal Overlap
- 05:40Signal to Noise (SNR) Characterization, Frequency Axis Calibration, and Calibration of Spectral Resolution
- 07:23EOM1 Modulation and Epi-Mode SRS Imaging
- 08:53Results: Optimizing Spectral Resolution, Two-Color SRS, and False Two-Color H&E Staining of SRS Images
- 10:14Conclusion
Stimulert Raman-spredning (SRS) mikroskopi er en kraftig, ikke-ødeleggende og etikettfri bildeteknikk. En fremvoksende applikasjon stimuleres Raman histologi, hvor tofarget SRS-avbildning ved protein- og lipid Raman-overgangene brukes til å generere pseudo-hematoksylin- og eosinbilder. Her demonstrerer vi en protokoll for sanntids, tofarget SRS-avbildning for vevsdiagnose.
Tags
Real-timeTwo-colorStimulated Raman ScatteringImagingMouse BrainSpectral FocusingSRS MicroscopyHistologySmall MoleculesDrugsCellsAchromatic LensesPump BeamLaser BeamDichroic MirrorSteering MirrorsScan MirrorsPhotodiodeLow Pass FiltersBeam SamplerFast PhotodiodeBand Pass FilterRF AttenuatorPhase Shifter
