Journal
/
Biologie
/
Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses

Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses

DOI:
10.3791/63487-v

•

09:55 min

•

February 03, 2022

•

, , , ,
1Brain-Immune Communication Lab,Institut Pasteur, 2Cytometry platform, CB UTechS,Institut Pasteur

Chapitres

  • 00:05Introduction
  • 00:42Dissection of the Choroid Plexus from the Brain
  • 02:47Preparation of Samples for Flow Cytometry Analysis
  • 03:52Choroid Plexus Myeloid Cells Gating
  • 06:46Choroid Plexus T Cells Gating
  • 07:44Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
  • 09:24Conclusion

Summary

Traduction automatique

Traduction automatique

This study uses flow cytometry and two different gating strategies on isolated perfused mice brain choroid plexuses; this protocol identifies the main immune cell subsets that populate this brain structure.

Tags

Keywords: Immune CellsChoroid PlexusesMouse ModelBrain RegulationCell DissectionCell IsolationCell CharacterizationCollagenase IVLateral VentricleThird VentricleFourth VentricleCell DissociationMACS BufferCell CentrifugationCell Washing

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/63487?article_type=v

Isolation of Retinal Stem Cells from the Mouse Eye
check_url/fr/63487?article_type=v

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
check_url/fr/63487?article_type=v

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
check_url/fr/63487?article_type=v

Isolation of Immune Cells from Primary Tumors
check_url/fr/63487?article_type=v

Generation of Mice Derived from Induced Pluripotent Stem Cells
check_url/fr/63487?article_type=v

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
check_url/fr/63487?article_type=v

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
check_url/fr/63487?article_type=v

Isolation and Characterization of Microvesicles from Peripheral Blood
check_url/fr/63487?article_type=v

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
check_url/fr/63487?article_type=v

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Read Article