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Immunologie et infection
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分析易患狼疮的MRL/lpr小鼠的蛋白尿、白细胞肾浸润和蛋白质的肾脏沉积
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Immunologie et infection
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Journal JoVE Immunologie et infection
Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
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分析易患狼疮的MRL/lpr小鼠的蛋白尿、白细胞肾浸润和蛋白质的肾脏沉积

DOI:
10.3791/63506-v

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09:43 min

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June 08, 2022

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1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Chapitres

  • 00:05Introduction
  • 00:27Proteinuria Test
  • 01:17Isolation of the Kidney Cells
  • 04:51Immunofluorescent Staining
  • 07:39Results: Characterizing Lupus Nephritis Based on Cell Infiltration and Protein Deposition in the Kidneys
  • 09:02Conclusion

Summary

Traduction automatique

Traduction automatique

本方案描述了一种跟踪小鼠狼疮进展的方法。提出了另外两种程序来表征基于细胞浸润和肾脏中蛋白质沉积的狼疮性肾炎。

Tags

ProteinuriaRenal InfiltrationLeukocytesRenal DepositionProteinsLupus-prone MRL/lpr MiceChronic InflammationKidneysLupusUrine CollectionKidney DissectionCell IsolationPercoll GradientLeukocyte PurificationFlow Cytometry

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