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Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion
Journal JoVE
Biologie
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Journal JoVE Biologie
Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion

Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion

DOI:
10.3791/63529-v

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09:56 min

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February 11, 2022

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1Department of Cell, Developmental, and Integrative Biology,University of Alabama at Birmingham

Chapitres

  • 00:04Introduction
  • 00:47Surface Preparation
  • 04:25Cell Preparation and Staining
  • 06:28Image Acquisition
  • 07:43Results: Effect of Ligand-Activated EGFR on Integrin-Mediated Cell Mechanics and Adhesion Formation in Cos-7 Cells
  • 09:11Conclusion

Summary

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Traduction automatique

TGT surface is an innovative platform to study growth factor-integrin crosstalk. The flexible probe design, specificity of the adhesion ligand, and precise modulation of stimulation conditions allow robust quantitative assessments of EGFR-integrin interplay. The results highlight EGFR as a 'mechano-organizer' tuning integrin mechanics, influencing focal adhesion assembly and cell spreading.

Tags

Keywords: Tension Gauge TetherIntegrin MechanicsCell AdhesionEGFR-Integrin CrosstalkSurface FunctionalizationAPTES SilanizationNHS-BiotinCell MechanicsBioengineering Protocols

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