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Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells
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Journal JoVE Biologie
Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells

Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells

DOI:
10.3791/63585-v

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07:49 min

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June 02, 2022

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, ,
1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences,Sun Yat-sen University

Chapitres

  • 00:04Introduction
  • 00:47LGSC Primary Culture
  • 03:43LGSC Maintenance and Passage
  • 04:48LGSC Differentiation
  • 05:34Results: Characterization, Differentiation, and Engraftment of LGSCs and Relief of ADDED Symptoms
  • 07:08Conclusion

Summary

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Traduction automatique

The three-dimensional, serum-free culture method for adult lacrimal gland (LG) stem cells is well established for the induction of LG organoid formation and differentiation into acinar or ductal-like cells.

Tags

Keywords: Lacrimal GlandStem CellsThree-dimensionalSerum-freeCulture SystemRegenerationReconstructionExpansion CapacityDifferentiationEuthanized MouseDispaseCollagenase ITrypsin EDTADulbecco’s Modified Eagles MediumHam’s F-12Matrix GelLacrimal Gland Stem Cell Medium

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