Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit

Journal

Biologie

Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit

Journal JoVE
Biologie

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Journal JoVE Biologie

Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit

DOI:

10.3791/636-v

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12:39 min

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January 22, 2008

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Linda Doan, Edwin S. Monuki

¹Department of Developmental and Cell Biology,University of California, Irvine (UCI)

Chapitres

00:00Titre
00:12Introduction
01:04Preparing Tissue and PCR Reaction Mixture
06:58Real Time Quantitative PCR
11:10Melting Curve Analysis
11:45Conclusion

Summary

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The complete genotyping of a mouse tail sample, including tissue digestion and PCR readout, is done in one and a half hours using Sigma's SYBR Green Extract-N-Amp Tissue PCR kit.

Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit

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Chapitres

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