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Organoïdes intestinaux canins dans un système de soutien perméable à double chambre
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1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Office of New Animal Drug Evaluation,Center for Veterinary Medicine, Food and Drug Administration, 5Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences,Center for Drug Evaluation and Research, Food and Drug Administration
Chapitres
- 00:04Introduction
- 01:05Permeable Support Seeding
- 05:30TEER Value Measurement
- 06:51Experimental Layout
- 08:09Results: Staining, Light Microscopy, and TEM Images of Canine Intestinal Organoid Monolayer
- 10:36Conclusion
Nous présentons ici un protocole décrivant la culture d’organoïdes intestinaux canins dans un système de soutien perméable à double chambre. L’ensemencement organoïde dans les supports perméables, l’entretien monocouche et les expériences ultérieures de perméabilité aux médicaments sont décrits.
Tags
Canine Intestinal OrganoidsDual-chamber Permeable Support SystemBiomedical Research ModelsOral PermeabilityTherapeutic Drug CandidatesIn Vitro Tool2D Cell Lines3D Self-assembled Epithelial StructuresAdult Stem CellsMonolayer MaintenanceExtracellular MatrixTight JunctionsCell DifferentiationNuclear ReceptorsCaco-2MDCKGastrointestinal AnatomyIntestinal MicrofloraDrug PermeabilityParallel Drug Development
