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Isolering, utvidelse og differensiering av mesenkymale stamceller fra Infrapatellar Fat Pad of the Goat Stifle Joint
Journal JoVE
Bio-ingénierie
Author Produced
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Journal JoVE Bio-ingénierie
Isolation, Expansion, and Differentiation of Mesenchymal Stem Cells from the Infrapatellar Fat Pad of the Goat Stifle Joint
DOI:

12:06 min

August 02, 2022

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Chapitres

  • 00:05Introduction
  • 01:08Isolation of Infrapatellar Fat Pad Mesenchymal Stem Cells (IFP-MSCs) from the Femorotibial Joint of Goat Knee
  • 05:16Maintenance and Expansion of Isolated Cells
  • 07:13Differentiation Potential of IFP-MSCs
  • 08:49Results: Expansion, Clonogenic Ability, and Differentiation Potential of IFP-MSCs
  • 11:07Conclusion

Summary

Traduction automatique

Infrapatellar fettpute mesenkymale stamceller (IFP-MSCs) kan enkelt isoleres fra kneleddets infrapatellar fettpute. De sprer seg godt in vitro, danner CFU-F-kolonier og skiller seg ut i adipogene, kondrogene og osteogene linjer. Her er metodikken for isolering, utvidelse og differensiering av IFP-MSCs fra geitkvelerfeste gitt.

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