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Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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Journal JoVE Biologie
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

DOI:
10.3791/63629-v

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10:07 min

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March 16, 2022

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, , , , , , ,
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences,Henan University, 2Research Institute of Applied Biology,Shanxi University, 3School of Life Science,Shanxi University

Chapitres

  • 00:04Introduction
  • 00:43Microinjection and Culture of the Eggs
  • 04:01Mutation Rate Estimation and Screening of Mutants
  • 06:08Establishment and Passaging of Mutant Lines and Egg Cryopreservation and Resuscitatio
  • 07:48Results: CRISPR/Cas9 Ribonuclease-Mediated Construction of Homozygous Locust Mutants
  • 09:19Conclusion

Summary

Traduction automatique

Traduction automatique

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

Tags

Keywords: CRISPR/Cas9Homozygous MutantsMigratory LocustAgricultural PestGene EditingMicroinjectionEgg CollectionMutant ScreeningHomozygous MaintenanceRibonucleoproteinInjection NeedleEgg TanningInjection ParametersIncubation

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