Journal

Biochimie

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Journal JoVE
Biochimie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biochimie

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Please note that all translations are automatically generated. Click here for the English version.

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

DOI:

10.3791/63668-v

•

11:38 min

•

April 05, 2022

•

Sohee Choi, Jessica A. San Juan, Marie C. Heffern, Michael J. Stevenson

¹Department of Chemistry,University of San Francisco, ²Department of Chemistry,University of California, Davis

Chapitres

00:04Introduction
00:59Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition
02:44Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand
03:56Isothermal Titration Calorimetry
09:00Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins
10:48Conclusion

Summary

Traduction automatique

This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

•

•

•

Chapitres

Summary

Traduction automatique

Tags

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

自动声胶肽受体激动剂的效价测定测定的连续稀释

使用脚手架脂质体重构脂质近端蛋白质 - 蛋白质相互作用<em>体外</em

时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

高亲和力抗体 - 抗原的结合动力学的测定，使用四个生物传感器平台

用耐热配体测量生物分子 DSC 剖面快速表征折叠和结合的相互作用

个性化肽阵列在器官移植 HLA 抗体检测中的研究

用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性

膜蛋白对洗涤剂敏感相互作用的检测

用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法

高速离心条件下 u937 细胞的细胞分裂

Read Article