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Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses
 

Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses

Article DOI: 10.3791/63686-v • 07:56 min • June 8th, 2022
June 8th, 2022 •
1, 1, 2, 1, 1, 3, 4, 1, 1
1Department of Physiology, Faculty of Medical Science and Center of Excellence for Innovation in Chemistry, Naresuan University, 2Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, 3Cosmetics and Natural Products Research Center, Faculty of Pharmaceutical Sciences, Naresuan University, 4Snyder Institute for Chronic Diseases, Airways Inflammation Research Group, Department of Physiology and Pharmacology, University of Calgary

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Vascular responses of arterial pulmonary circulation can be explored using intrapulmonary artery (IPA) and vascular smooth muscle cells (VSMCs). The present study describes the isolation of IPA in detail and the protocols used for investigating vasorelaxation in response to physiological stimuli.

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Keywords: Intrapulmonary Artery Vascular Smooth Muscle Cells Organ Bath Technique Vascular Physiology Pulmonary Hypertension Papain Collagenase Cell Isolation
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